Influence of heavy metals on physiological and biochemical parameters of Pseudorasbora parva (Cypriniformes, Cyprinidae)

Anthropogenic load on aquatic ecosystems leads to increased inputs of heavy metals, which can have a toxic effect organisms. Some the most appropriate objects for research are short-cycle fish species. This article considers results studies adaptive reactions stone moroko Pseudorasbora parva (Temminck et Schlegel, 1846) Mn, Pb, Ni metal ions, exceeded reference values in reservoir by 1.7, 1.5 and 2.0 times, respectively. Changes morphological parameters blood histocytological pattern hepatopancreas experimental species under influence toxicants, as well changes biochemical parameters, were determined. It was noted that Mn caused pathological form poikilocytosis. The morphometric erythrocytes (the cell area nuclear area) reached 67.48 ± 0.67 13.97 0.22 µm2 respectively (4.0% 13.8% less compared control). resulted an number leukocytes immature forms erythrocytes. 0.9% smaller than control group, nucleus 4.5% control. Pb consisted red cells nuclei 6.5% 8.3%, respectively, percentage white exposed tended increase. In experiment with nickel, 10.2% formed elements; lead – 11.3%; manganese 6.1%, while control, total elements only 1.2%. Cytometric revealed there significant differences shape, size, location hepatocytes different fish. structural components liver histological specimens stained differentially intensities colours. ions did not clearly defined boundaries, large destroyed cells, indicates these metals. had largest highest nuclear-cytoplasmic ratio is typical Mn. size occupied 12.7% internal contents cells. shown impact studied concentrations lactate dehydrogenase activity 1.22, 1.14 1.48 times contrast, 3.27-fold decrease succinate Besides that, enzyme decreased 1.68 action Pb. Subsequently, we found increase alkaline phosphatase muscle tissue 3.25–3.94 toxicants. Muscle protein levels lower distinct (1.53 times). obtained data physiological metals provide opportunity predict composition fauna conditions excessive pollution ecosystems.